Graduate NIH Training in BiotechnologyFormer TraineesNathan Domagalski
Nathan completed his training program August 2005. He is currently employed as a Research Investigator 2 at Bristol-Myers Squibb Company in the area of pharmaceutical process research and development. Project Description Research by our group has experimentally demonstrated that Escherichia coli (E. coli) may be genetically modified to increase recombinant protein production by eliminating a metabolic pathway. This modification also decreases acetate production. Although the gram-negative microorganism E. coli is commonly used in bulk fermentations, the gram-positive microorganism Bacillus subtilis (B. subtilis) offers advantages of reduced toxicity since it lacks lipopolysaccharide (LPS) in the cell wall. Working with an inherently safer microorganism expands the usefulness of our E. coli discoveries. Therefore, the goal of our recent research is to increase recombinant protein production in B. subtilis by following the strategies learned while working with E. coli. Courses 06608A SAFETY IN SCIENCE/ENGINEERING 06703A ADV FLUID DYNAMICS 06705A ADV CHE THERMODYNAMICS 06713A MATHEMATICAL TECHNIQUES IN CHEMICAL ENGINEERING 06717A BIOTECH & ENVIRONMENTAL PROCESSES 06702A ADV KINETICS 06704A ADV HEAT AND MASS TRANSFER PT708A INTRO TO MOLECULAR BIOLOGY PT737A FUNDAMENTALS OF BIOMEDICAL SCIENCE Publications Engineering of Bacillus subtilis for the Enhanced Total Synthesis of Folic Acid, T. Zhu, Z. Pan, N. Domagalski, R. Koepsel, M.M. Ataai, and M.M. Domach, In Press, APPLIED & ENV MICROBIOL Historical Perspective and Basics of Molecular Biology, N.R. Domagalski and MM Domach, CRC Handbook of Biomedical Engineering, In Press Comparison of Acetate Minimization Strategies for E. coli and Effect on Green Fluorescent Protein Production, N. Domagalski R. Koepsel, M.M. Ataai, and M.M. Domach , Submitted Enhanced Heterologous Protein Production and Acetate Minimization in B. subtilis, N. Domagalski, R. Koepsel, M.M. Ataai, and M.M. Domach, Submitted Presentations Domagalski, N., T. Zhu, R. Koepsel, M. M. Ataai, and M. M. Domach. Protein expression in cells metabolically engineered to have diminished acetate production. American Chemical Society. National Meeting, March 2004. Anaheim, CA. Domagalski, N., Z. Pan, T. Zhu, K. Ye, R. Koepsel, M. M. Ataai, and M. M. Domach. Combining enhanced traits of E. coli into B. subtilis. American Institute of Chemical Engineers. Annual Meeting, November 2004. Austin, TX. Advisor Michael M. Domach Professor of Chemical Engineering Carnegie Mellon University
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