Graduate NIH Training in BiotechnologyTrainees
Drew S. Cunningham
Project Description Current efforts are aimed at using metabolic engineering and molecular biology methods to develop bacteria that maximize the conversion of a given carbon feed into biomass and desired products, as opposed to generating by-products that are not only wastes of carbon, but also toxic to the cell. Specifically, we have been working with the Gram-negative Escherichia coli and the Gram-positive Bacillus subtilis, trying in each model organism to minimize the production of the toxic by-product, acetate, in efforts to re-route some of the otherwise wasted carbon into production of desired heterologous recombinant protein products. Specific strategies that seek to maximize recombinant protein production and/or minimize acetate by-production that are being examined at present include pyruvate kinase knockouts and the use of designed media. Bio Classes Introduction to Cell & Molecular Biology Biochemistry for Engineers Foundations of Biomedical Sciences Foundations of Biomedical Sciences Conference Biochemistry Genetics Fundamentals of Biochemical Engineering Publications 1st Publication Ready for submission: Supply-Side Comparison of Stable Heterologous Protein Expression in Pyruvate Kinase-Deficient & Wild-Type E. coli, Authors: D. S. Cunningham, N. Domagalski, R. Koepsel, M. M. Ataai, and M. M. Domach Presentations None Seminars None
Advisor
Michael M. Domach |
Biotechnology Program
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